Journal: Molecular Neurobiology
Article Title: Tauroursodeoxycholic Acid Inhibited Apoptosis and Oxidative Stress in H 2 O 2 -Induced BMSC Death via Modulating the Nrf-2 Signaling Pathway: the Therapeutic Implications in a Rat Model of Spinal Cord Injury
doi: 10.1007/s12035-023-03754-5
Figure Lengend Snippet: The combined treatment of TUDCA and BMSC transplantation accelerated the regeneration of tissues and motor functional recovery after SCI. a Images of behavioral observation showing the characteristics of hind limb movement of rats across different groups. b Footprint analyses of different groups on day 28 after SCI. c Representative images from H&E staining in longitudinal section on day 28 after SCI. d , e The survived neurons following Nissl staining and relative quantification at the lesion area from different groups on day 28 after SCI. f The Basso, Beattie, and Bresnahan (BBB) locomotor scores of different groups. g The Immunofluorescence staining of MAP2(green), MBP (green), TUJ1 (red), and GFAP (red) from different groups on day 28 post-SCI. Data are presented as mean ± SD. * P < 0.05 vs. the SHAM group. # P < 0:05 vs. the SCI group. & P < 0.05 vs. the BMSC group ( n = 6)
Article Snippet: According to a previous study [ ], the tissues were subjected to overnight incubation at the temperature of 4 °C with microtubule-associated protein 2 (MAP2) (1:200, Boster Biological Engineering Co.), glial scar formation (GFAP) (1:200, Boster Biological Engineering Co.), myelin basic protein (MBP) (1:200, Boster Biological Engineering Co.), and beta-tubulin III (TUJ1) (1:200, CST) as primary antibodies.
Techniques: Transplantation Assay, Functional Assay, Staining, Quantitative Proteomics, Immunofluorescence
